Source: Rice Grain (Oryza Sativa)
Catalog No: HYC002M01
Introduction:
Human serum albumin is the most abundant protein in human plasma. It can transport hormones, lipids and other molecules and maintain osmotic pressure. OsrHSA is a recombinant human serum albumin derived from rice grains, which provides an excellent solution for animal free medium. Compared to FBS, pHSA and BSA, OsrHSA has higher purity and excellent batch consistency. Meanwhile, OsrHSA is widely used as excipient, stabilizer and embedding agent in bio-pharmaceutical applications.
Physical Appearance: Off-white to light beige lyophilized powder
Formulation: It is lyophilized with saline.
Purity: More than 99% as determined by SDS-PAGE and HPLC analysis.
Endotoxin: Less than 0.125EU/mg
Applications:
Mammalian cell culture
Vaccine manufacture and formulation
In vivo/vitro diagnostics
Cryopreservation of cell
Coating of medical devices
Cosmetic usage
Storage and Handling:
Stored at temperatures 2-8℃ for 24 months. For lyophilized powder, it is recommended to reconstitute the lyophilized OsrHSA in sterile PBS. Upon reconstitution OsrHSA should be stored below -20℃. Use as soon as possible after opening. Please avoid freeze-thaw cycles.
1.SDS-PAGE
Batches consistency of seven batches of OsrHSA. It presentes in SDSPAGE and visualized by silver staining, showing a single band at 66.5kDa.
2. Comparison with commercial rHSA
Compared with the commercial rHSA, OsrHSA has no degraddation fragment and fewer polymers.
3. OsrHSA keeps iPS cells in an undifferentiated state
The immunofluorescent staining showed OsrHSA was effective in keeping undifferentiation and keeping viability of iPS cells.
4. Performance of OsrHSA in promoting cell growth and antibody production
The promotion of cell growth was comparable to that of CHO cells on 10% FBS. OsrHSA showed a 20% increase in maximum viability and density compared with pHSA in Vero cells. The titer of antibody (IgG1+κ) measured SP2/0 cells supplemented with OsrHSA was significantly higher than that supplemented with pHSA.
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