Recombinant Protein Expression
Source:Posted by Themecurve| Time:2020-04-29| Hits:241

Rice endosperm cell specific and efficient expression technology platform for proteins and polypeptide expression of technical services, to achieve a stable and efficient expression of the purpose of protein gene engineering rice lines, can also provide the purpose of different levels of protein and peptide purification process development. 

 

Phase

Service

Phase 1: 

Generation of genetic engineering plants

Gene optimization and synthesis, vector construction

Generation of transgenic plants (T0, 20~50 positive plants)

Screening for high expression lines

Phase 2: Lab scale 

Screening the genetic stability of homozygous genetic engineering lines

Detection and activity analysis of recombinant protein

Developing a protocol for purification of the protein and providing a microgram scale with 90-95% purity

Phase 3: Pilot scale

Providing the milligram or gram protein with 90%~95% purity

Phase4: Large scale

Developing a protocol of large processing and manufacturing

 

Provide the rice endosperm expression technology to expression customized proteins

  • Target protein expression (100~1000 amino acids)
  • Small molecular polypeptides (less than 100 amino acids)
  • Expression level reaches 0.1~0.9% of brown rice dry weight

 

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