Recombinant Protein Expression
Rice endosperm cell specific and efficient expression technology platform for proteins and polypeptide expression of technical services, to achieve a stable and efficient expression of the purpose of protein gene engineering rice lines, can also provide the purpose of different levels of protein and peptide purification process development.
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Phase |
Service |
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Phase 1: Generation of genetic engineering plants |
Gene optimization and synthesis, vector construction |
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Generation of transgenic plants (T0, 20~50 positive plants) |
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Screening for high expression lines |
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Phase 2: Lab scale |
Screening the genetic stability of homozygous genetic engineering lines |
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Detection and activity analysis of recombinant protein |
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Developing a protocol for purification of the protein and providing a microgram scale with 90-95% purity |
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Phase 3: Pilot scale |
Providing the milligram or gram protein with 90%~95% purity |
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Phase4: Large scale |
Developing a protocol of large processing and manufacturing |
Provide the rice endosperm expression technology to expression customized proteins
1）Target protein expression (100~1000 amino acids)
2）Small molecular polypeptides (less than 100 amino acids)
3）Expression level reaches 0.1~0.9% of brown rice dry weight
